Glycolysis and Lactate Dehydrogenase A in Iron-Mediated Suppression of Osteoblast Function

Authors

  • Pete Hunter Indiana University School of Medicine
  • Daniel Edwards Indiana University School of Medicine
  • Christopher Miller Indiana University School of Medicine
  • Erica Clinkenbeard Indiana University School of Medicine

DOI:

https://doi.org/10.18060/24638

Abstract

Background/Objective 

An estimated 37 million Americans have Chronic Kidney Disease (CKD), a condition in characterized by gradual decline in kidney function1. Patients with CKD are often afflicted with skeletal fractures, increasing morbidity and mortality. CKD has also shown a strong correlation with iron-deficiency anemia. The underlying mechanisms of how iron-deficiency anemia of CKD affects bone loss are not well understood. Based on RNA-sequencing results, we hypothesize that lactate dehydrogenase A (LDHA) may play a role in iron-deficiency mediated suppression of osteoblast differentiation and function. 

 

Methods 

Mouse Progenitor Cells (MPC2) were incubated in osteogenic media along with deferoxamine (DFO) to induce differentiation in chronic iron deficiency; samples were collected after 7 and 14 days. Quantitative real-time PCR and western blot were used to validate LDHA mRNA and protein levels in DFO treated MPCs versus control cells. RNA levels of osteoblast genes and LDHA were also assessed in a pre-clinical mouse model of CKD. 

 

Results:  

In vitro, MPCs cultured in DFO media showed a significant increase of LDHA mRNA at 7 days (p=0.015) and returned to near control levels by day 14. Western blots showed a slight increase of total LDHA protein in DFO treated MPCs at 7 days and a large increase of protein at the 14-day mark (p=0.051). In vivo, CKD bone marrow showed a reduction in osteoblast gene expression (osteocalcin and type 1 collagen; p<0.05). LDHA mRNA expression was increased in CKD mice bone marrow when compared to wild-type mice (p=0.051), suggesting an inverse relationship. 

 

Conclusion and Potential Impact 

Inappropriate activation of glycolysis and LDHA appears to play a role in iron-deficiency mediated suppression of osteoblast function in relation to CKD, both in vitro and in vivo. Further exploration of this relationship could be critical to the development of improved treatment options to maintain bone homeostasis during CKD. 

Author Biographies

Pete Hunter, Indiana University School of Medicine

Department of Medical and Molecular Genetics  

 

Daniel Edwards, Indiana University School of Medicine

Department of Medical and Molecular Genetics  

 

Christopher Miller, Indiana University School of Medicine

Department of Medical and Molecular Genetics  

 

Erica Clinkenbeard, Indiana University School of Medicine

Department of Medical and Molecular Genetics  

 

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Published

2020-12-15

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Abstracts